6jy8

Structure of dark-state marine bacterial chloride importer, NM-R3, with CW laser (ND-3%) at 95K.

spinqualitylabelsall models 6jy8, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6jy8 is a 1 chain structure with sequence from Nonlabens marinus S1-08. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: CL, OLA, RET Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function W8VZW3_9FLAO Publication Abstract from PubMed A newly identified microbial rhodopsin, NM-R3, from the marine flavobacterium Nonlabens marinus, was recently shown to drive chloride ion uptake, extending our understanding of the diversity of mechanisms for biological energy conversion. To clarify the mechanism underlying its function, we characterized the crystal structures of NM-R3 in both the dark state and early intermediate photoexcited states produced by laser pulses of different intensities and temperatures. The displacement of chloride ions at five different locations in the model reflected the detailed anion-conduction pathway, and the activity-related key residues-Cys(105), Ser(60), Gln(224), and Phe(90)-were identified by mutation assays and spectroscopy. Comparisons with other proteins, including a closely related outward sodium ion pump, revealed key motifs and provided structural insights into light-driven ion transport across membranes by the NQ subfamily of rhodopsins. Unexpectedly, the response of the retinal in NM-R3 to photostimulation appears to be substantially different from that seen in bacteriorhodopsin. Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family.,Yun JH, Ohki M, Park JH, Ishimoto N, Sato-Tomita A, Lee W, Jin Z, Tame JRH, Shibayama N, Park SY, Lee W Sci Adv. 2020 Feb 7;6(6):eaay2042. doi: 10.1126/sciadv.aay2042. eCollection 2020 , Feb. PMID:32083178 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Rhodopsin 3D structures

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