6lcj

TtGalA, alpha-galactosidase from Thermus thermopilus in apo form

spinqualitylabelsall models 6lcj, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6lcj is a 6 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q53W51_THET8 Publication Abstract from PubMed alpha-Galactosidase catalyzes the hydrolysis of a terminal alpha-galactose residue in galacto-oligosaccharides and has potential in various industrial applications and food processing. We determined the crystal structures of alpha-galactosidase from the thermophilic microorganism Thermus thermophilus (TtGalA) and its complexes with pNPGal and stachyose. The monomer folds into an N-terminal domain, a catalytic (beta/alpha)8 barrel domain, and a C-terminal domain. The domain organization is similar to that of the other family of 36 alpha-galactosidases, but TtGalA presents a cagelike hexamer. Structural analysis shows that oligomerization may be a key factor for the thermal adaption of TtGalA. The structure of TtGalA complexed with stachyose reveals only the existence of one -1 subsite and one +1 subsite in the active site. Structural comparison of the stachyose-bound complexes of TtGalA and GsAgaA, a tetrameric enzyme with four subsites, suggests evolutionary divergence of substrate specificity within the GH36 family of alpha-galactosidases. To the best of our knowledge, the crystal structure of TtGalA is the first report of a quaternary structure as a hexameric assembly in the alpha-galactosidase family. Crystal Structure of alpha-Galactosidase from Thermus thermophilus: Insight into Hexamer Assembly and Substrate Specificity.,Chen SC, Wu SP, Chang YY, Hwang TS, Lee TH, Hsu CH J Agric Food Chem. 2020 Jun 3;68(22):6161-6169. doi: 10.1021/acs.jafc.0c00871., Epub 2020 May 20. PMID:32390413 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Galactosidase 3D structures

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