6lod

Cryo-EM structure of the air-oxidized photosynthetic alternative complex III from Roseiflexus castenholzii

spinqualitylabelsall models 6lod, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6lod is a 6 chain structure with sequence from Roseiflexus castenholzii DSM 13941. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.2Å Ligands: ACE, EL6, HEC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A7NJ91_ROSCS Publication Abstract from PubMed Alternative complex III (ACIII) is a multisubunit quinol:electron acceptor oxidoreductase that couples quinol oxidation with transmembrane proton translocation in both the respiratory and photosynthetic electron transport chains of bacteria. The coupling mechanism, however, is poorly understood. Here, we report the cryo-EM structures of air-oxidized and dithionite-reduced ACIII from the photosynthetic bacterium Roseiflexus castenholzii at 3.3- and 3.5-A resolution, respectively. We identified a menaquinol binding pocket and an electron transfer wire comprising six hemes and four iron-sulfur clusters that is capable of transferring electrons to periplasmic acceptors. We detected a proton translocation passage in which three strictly conserved, mid-passage residues are likely essential for coupling the redox-driven proton translocation across the membrane. These results allow us to propose a previously unrecognized coupling mechanism that links the respiratory and photosynthetic functions of ACIII. This study provides a structural basis for further investigation of the energy transformation mechanisms in bacterial photosynthesis and respiration. Cryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii.,Shi Y, Xin Y, Wang C, Blankenship RE, Sun F, Xu X Sci Adv. 2020 Jul 29;6(31):eaba2739. doi: 10.1126/sciadv.aba2739. eCollection , 2020 Jul. PMID:32832681 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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