6lu8

Cryo-EM structure of a human pre-60S ribosomal subunit - state A

spinqualitylabelsall models 6lu8, resolution 3.13Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6lu8 is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.13Å Ligands: 1MA, 2MG, 5MC, 5MU, 6MZ, 7MG, A2M, B8H, B8K, B8Q, B8T, B8W, B9B, B9H, BGH, E6G, E7G, I4U, M7A, MG, MHG, OMC, OMG, OMU, P4U, P7G, PSU, UR3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NOG2_HUMAN GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (PubMed:32669547). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed:26203195). Publication Abstract from PubMed Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors. Structural snapshots of human pre-60S ribosomal particles before and after nuclear export.,Liang X, Zuo MQ, Zhang Y, Li N, Ma C, Dong MQ, Gao N Nat Commun. 2020 Jul 15;11(1):3542. doi: 10.1038/s41467-020-17237-x. PMID:32669547 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Eukaryotic initiation factor 3D structures GTP-binding protein 3D structures Ribosome 3D structures Ribosome biogenesis protein 3D structures

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