Structural highlights
FunctionMFNA_METJA Specifically catalyzes the decarboxylation of L-tyrosine to produce tyramine.[1] Publication Abstract from PubMedPLP-dependent enzymes catalyze a plethora of chemical reactions affecting diverse physiological functions. Here we report the structural determinants of the reaction mechanism in a Group II PLP-dependent decarboxylase by assigning two early intermediates. The in-crystallo complexes of the PLP bound form, and the Dunathan and quinonoid intermediates, allowed direct observation of the active site interactions. The structures reveal that a subtle rearrangement of a conserved Arg residue in concert with a water-mediated interaction with the carboxylate of the Dunathan intermediate, appears to directly stabilize the alignment and facilitate the release of CO2 to yield the quinonoid. Modeling indicates that the conformational change of a dynamic catalytic loop to a closed form controls a conserved network of hydrogen bond interactions between catalytic residues to protonate the quinonoid. Our results provide a structural framework to elucidate mechanistic roles of residues that govern reaction specificity and catalysis in PLP-dependent decarboxylation. Crystallographic Snapshots of the Dunathan and Quinonoid Intermediates provide Insights into the Reaction Mechanism of Group II Decarboxylases.,Gayathri SC, Manoj N J Mol Biol. 2020 Dec 4;432(24):166692. doi: 10.1016/j.jmb.2020.10.026. Epub 2020 , Oct 27. PMID:33122004[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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