6mcj

Structure of Helical Carotenoid Protein 2 from Fremyella diplosiphon

spinqualitylabelsall models 6mcj, resolution 1.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6mcj is a 2 chain structure with sequence from Tolypothrix sp. PCC 7601. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.712Å Ligands: 1PE, 45D, IOD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0D6L0Q7_9CYAN Publication Abstract from PubMed The Helical Carotenoid Proteins (HCPs) are a large group of newly identified carotenoid-binding proteins found in ecophysiologically diverse cyanobacteria. They likely evolved before becoming the effector (quenching) domain of the modular Orange Carotenoid Protein (OCP). The number of discrete HCP families-at least nine-suggests they are involved in multiple distinct functions. Here we report the 1.7A crystal structure of HCP2, one of the most widespread HCPs found in nature, from the chromatically acclimating cyanobacterium Tolypothrix sp. PCC 7601. By purifying HCP2 from the native source we are able to identify its natively-bound carotenoid, which is exclusively canthaxanthin. In solution, HCP2 is a monomer with an absorbance maximum of 530nm. However, the HCP2 crystals have a maximum absorbance at 548nm, which is accounted by the stacking of the beta1 rings of the carotenoid in the two molecules in the asymmetric unit. Our results demonstrate how HCPs provide a valuable system to study carotenoid-protein interactions and their spectroscopic implications, and contribute to efforts to understand the functional roles of this large, newly discovered family of pigment proteins, which to-date remain enigmatic. Structural and spectroscopic characterization of HCP2.,Dominguez-Martin MA, Polivka T, Sutter M, Ferlez B, Lechno-Yossef S, Montgomery BL, Kerfeld CA Biochim Biophys Acta Bioenerg. 2019 May 1;1860(5):414-424. doi:, 10.1016/j.bbabio.2019.03.004. Epub 2019 Mar 15. PMID:30880081 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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