6mu4

Bst DNA polymerase I FANA/DNA binary complex

spinqualitylabelsall models 6mu4, resolution 1.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6mu4 is a 3 chain structure with sequence from Geobacillus stearothermophilus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.62Å Ligands: A5L, CFL, GFL, MG, MPD, SO4, UF2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E1C9K5_GEOSE Publication Abstract from PubMed Replicative DNA polymerases are highly efficient enzymes that maintain stringent geometric control over shape and orientation of the template and incoming nucleoside triphosphate. In a surprising twist to this paradigm, a naturally occurring bacterial DNA polymerase I member isolated from Geobacillus stearothermophilus (Bst) exhibits an innate ability to reverse transcribe RNA and other synthetic congeners (XNAs) into DNA. This observation raises the interesting question of how a replicative DNA polymerase is able to recognize templates of diverse chemical composition. Here, we present crystal structures of natural Bst DNA polymerase that capture the post-translocated product of DNA synthesis on templates composed entirely of 2'-deoxy-2'-fluoro-beta-d-arabino nucleic acid (FANA) and alpha-l-threofuranosyl nucleic acid (TNA). Analysis of the enzyme active site reveals the importance of structural plasticity as a possible mechanism for XNA-dependent DNA synthesis and provides insights into the construction of variants with improved activity. Crystal structures of a natural DNA polymerase that functions as an XNA reverse transcriptase.,Jackson LN, Chim N, Shi C, Chaput JC Nucleic Acids Res. 2019 Jun 6. pii: 5512092. doi: 10.1093/nar/gkz513. PMID:31170294 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase 3D structures

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