6n2b
The Crystal Structure of Caldicellulosiruptor kristjanssonii Tapirin C-terminal domain
Structural highlights
FunctionPublication Abstract from PubMedGenomes of extremely thermophilic Caldicellulosiruptor species encode novel cellulose binding proteins, tapirins, located proximate to the type IV pilus locus. The C-terminal domain of a tapirin (Calkro_0844) from Caldicellulosiruptor kronotskyensis is structurally unique and has a cellulose binding affinity akin to family 3 carbohydrate binding modules (CBM3). Here, full-length and C-terminal versions of tapirins from Caldicellulosiruptor bescii (Athe_1870), Caldicellulosiruptor hydrothermalis (Calhy_0908), Caldicellulosiruptor kristjanssonii (Calkr_0826), and Caldicellulosiruptor naganoensis (NA10_0869) were produced recombinantly in Escherichia coli and compared to Calkro_0844. All five tapirins bound to microcrystalline cellulose, switchgrass, poplar, filter paper, but not to xylan. Densitometry analysis of bound protein fractions visualized by SDS-PAGE revealed that Calhy_0908 and Calkr_0826 (from weakly cellulolytic species) associated with the cellulose substrates to a greater extent than Athe_1870, Calkro_0844 and NA10_0869 (from strongly cellulolytic species). Perhaps this relates to their specific needs to capture glucans released from lignocellulose by cellulases produced in Caldicellulosiruptor communities. Calkro_0844, and NA10_0869 share a high degree of amino acid sequence identity (> 80% identity), more so than with Athe_1870 ( approximately 50%). The amino acid sequence identities of Calhy_0908 and Calkr_0826 compared to Calkro_0844 were only 16% and 36%, respectively, although the three-dimensional structures of their C-terminal binding regions were closely related. Unlike the parent strain, C. bescii mutants lacking the tapirin genes did not bind to cellulose following short-term incubation, suggesting a role in cell association with plant biomass. Given the scarcity of carbohydrates in neutral terrestrial hot springs, tapirins likely help scavenge carbohydrates from lignocellulose to support growth and survival of Caldicellulosiruptor species.IMPORTANCE Mechanisms by which microorganisms attach to and degrade lignocellulose are important to understand if effective approaches for conversion of plant biomass into fuels and chemicals are to be developed. Caldicellulosiruptor species grow on carbohydrates from lignocellulose at elevated temperatures and have biotechnological significance for that reason. Novel cellulose binding proteins, called tapirins, are involved in the way Caldicellulosiruptor species interact with microcrystalline cellulose and here additional information about the diversity of these proteins across the genus is provided, including binding affinity and three-dimensional structural comparisons. Comparative Biochemical and Structural Analysis of Novel Cellulose Binding Proteins (Tapirins) from Extremely Thermophilic Caldicellulosiruptor Species.,Lee LL, Hart WS, Lunin VV, Alahuhta M, Bomble YJ, Himmel ME, Blumer-Schuette SE, Adams MWW, Kelly RM Appl Environ Microbiol. 2018 Nov 26. pii: AEM.01983-18. doi:, 10.1128/AEM.01983-18. PMID:30478233[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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