6nmj

Crystal Structure of Rat Ric-8A G alpha binding domain, "Paratone-N Immersed"

spinqualitylabelsall models 6nmj, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6nmj is a 2 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B1H241_RAT Publication Abstract from PubMed Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Galpha). We report the 2.2-A crystal structure of the Ric-8A Galpha-binding domain with GEF activity, residues 1-452, and is phosphorylated at Ser435 and Thr440. Residues 1-429 adopt a superhelical fold comprised of Armadillo (ARM) and HEAT repeats, and the C terminus is disordered. One of the phosphorylated residues potentially binds to a basic cluster in an ARM motif. Amino acid sequence conservation and published hydrogen-deuterium exchange data indicate repeats 3 through 6 to be a putative Galpha-binding surface. Normal mode modeling of small-angle X-ray scattering data indicates that phosphorylation induces relative rotation between repeats 1-4, 5-6, and 7-9. 2D (1)H-(15)N-TROSY spectra of [(2)H,(15)N]-labeled Galphai1 in the presence of R452 reveals chemical shift perturbations of the C terminus and Galphai1 residues involved in nucleotide binding. Structure, Function, and Dynamics of the Galpha Binding Domain of Ric-8A.,Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR Structure. 2019 Jul 2;27(7):1137-1147.e5. doi: 10.1016/j.str.2019.04.013. Epub, 2019 May 30. PMID:31155309 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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