6o19

Crystal Structure of Pho7 complex with pho1 promoter site 2

spinqualitylabelsall models 6o19, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6o19 is a 3 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.596Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function YBCB_SCHPO Publication Abstract from PubMed Pho7 is the fission yeast Zn2Cys6 transcriptional factor that drives a response to phosphate starvation in which phosphate acquisition genes are upregulated. Here we report a crystal structure at 1.6 A resolution of the Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5'-TCGGAAATTAAAAA). Comparison to the previously reported structure of Pho7 DBD in complex with its binding site in the tgp1 promoter (5'-TCGGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DNA interface that accommodate different promoter DNA sequences. Mutagenesis of Pho7 amino acids at the DNA interface identified nucleobase contacts at the periphery of the footprint that are essential for the induction of pho1 expression in response to phosphate starvation and for Pho7 binding to site 1 in the pho1 promoter. Structure of fission yeast transcription factor Pho7 bound to pho1 promoter DNA and effect of Pho7 mutations on DNA binding and phosphate homeostasis.,Garg A, Goldgur Y, Sanchez AM, Schwer B, Shuman S Mol Cell Biol. 2019 Apr 22. pii: MCB.00132-19. doi: 10.1128/MCB.00132-19. PMID:31010807 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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