6p2i

Acyclic imino acid reductase (Bsp5) in complex with NADPH and D-Arg

spinqualitylabelsall models 6p2i, resolution 1.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6p2i is a 2 chain structure with sequence from Bacillus sp. 5mfcol3.1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.63Å Ligands: DAR, EDO, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1I4FUG4_9BACI Publication Abstract from PubMed Acyclic imines are unstable in aqueous conditions. For this reason, known imine reductases, which enable the synthesis of chiral amines, mainly intercept stable cyclic imines. Here we report the detailed biochemical and structural characterization of Bsp5, an imino acid reductase from the D-2-hydroxyacid dehydrogenase family that reduces acyclic imino acids produced in situ by a partner oxidase. We determine a 1.6 A resolution structure of Bsp5 in complex with D-arginine and coenzyme NADPH. Combined with mutagenesis work, our study reveals the minimal structural constraints for its biosynthetic activity. Furthermore, we demonstrate that Bsp5 can intercept more complex products from an alternate oxidase partner, suggesting that this oxidase-imino acid reductase pair could be evolved for biocatalytic conversion of L-amino acids to D-amino acids. An Asymmetric Reductase that Intercepts Acyclic Imino Acids Produced In Situ by a Partner Oxidase.,Guo J, Higgins MA, Daniel-Ivad P, Ryan KS J Am Chem Soc. 2019 Jul 12. doi: 10.1021/jacs.9b03307. PMID:31298853 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA