6pwq

Crystal structure of Levansucrase from Bacillus subtilis mutant S164A at 2.6 A

spinqualitylabelsall models 6pwq, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6pwq is a 2 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: CA, GOL, PG4, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SACB_BACSU Publication Abstract from PubMed Mutation S164A largely affects the transfructosylation properties of Bacillus subtilis levansucrase (SacB). The variant uses acceptors such as glucose and short levans with an average molecular weight of 7.6 kDa more efficiently than SacB, leading to the enhanced synthesis of medium and high molecular weight polymer and a blasto-oligosaccharide series with a polymerization degree of 2-10. A 3-fold increase in blasto-oligosaccharides yield is provoked by the modified interplay between the variant and glucose. Despite its modified product specificity, protein-carbohydrate and protein-protein interactions are still a major factor affecting size and distribution of levan molecular weight. This study highlights the importance of critical factors such as protein concentration in the analysis of wild-type and mutagenized levansucrases. Docking experiments with the crystal structures of SacB and variant S164A - the latter obtained at a 2.6 A resolution - identified unreported potential binding subsites for fructosyl moieties on the surface of both enzymes. Implications of the mutation S164A on Bacillus subtilis levansucrase product specificity and insights into protein interactions acting upon levan synthesis.,Ortiz-Soto ME, Porras-Dominguez JR, Rodriguez-Alegria ME, Morales-Moreno LA, Diaz-Vilchis A, Rudino-Pinera E, Beltran-Hernandez NE, Rivera HM, Seibel J, Lopez Munguia A Int J Biol Macromol. 2020 Jun 15;161:898-908. doi:, 10.1016/j.ijbiomac.2020.06.114. PMID:32553967 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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