6qnz | pdb_00006qnz

Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant

spinqualitylabelsall models 6qnz, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6qnz is a 2 chain structure with sequence from Bacillus sp. D6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.45Å Ligands: GOL, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A3FEV7_9BACI Publication Abstract from PubMed The mutated nickase Nt.BspD6I E418A has been obtained by site-directed mutagenesis. The purified protein has been crystallized, and its spatial structure has been determined at 2.45 A resolution. An analysis of the crystal structures of the wild-type and mutated nickase have shown that the elimination of a carboxyl group due to the E418A mutation initiates marked conformational changes in both the N-terminal recognition domain and the C-terminal catalytic domain of nickase and insignificantly affects its linker domain. This is supported by changes in the functional properties of mutated nickase: an increase in the oligomerization capacity in the presence of a substrate, a reduction in the capacity to bind a substrate, and complete loss of catalytic activity. The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant.,Artyukh RI, Kachalova GS, Yunusova AK, Fatkhullin BF, Atanasov BP, Perevyazova TA, Popov AN, Gabdulkhakov AG, Zheleznaya LA J Struct Biol. 2020 Apr 13:107508. doi: 10.1016/j.jsb.2020.107508. PMID:32298813 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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