6qva

Crystal structure of a CHAD domain from Chlorobium tepidum in complex with inorganic polyphosphate

spinqualitylabelsall models 6qva, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6qva is a 1 chain structure with sequence from Chlorobaculum tepidum TLS. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.05Å Ligands: 3PO, 9PI, EDO, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8KE09_CHLTE Publication Abstract from PubMed Inorganic polyphosphates (polyPs) are linear polymers of orthophosphate units linked by phosphoanhydride bonds. Here, we report that bacterial, archaeal, and eukaryotic conserved histidine alpha-helical (CHAD) domains are specific polyP-binding modules. Crystal structures reveal that CHAD domains are formed by two four-helix bundles, giving rise to a central pore surrounded by conserved basic surface patches. Different CHAD domains bind polyPs with dissociation constants ranging from the nano- to mid-micromolar range, but not nucleic acids. A CHAD-polyP complex structure reveals the phosphate polymer binding across the central pore and along the two basic patches. Mutational analysis of CHAD-polyP interface residues validates the complex structure. The presence of a CHAD domain in the polyPase ygiF enhances its enzymatic activity. The only known CHAD protein from the plant Ricinus communis localizes to the nucleus/nucleolus when expressed in Arabidopsis and tobacco, suggesting that plants may harbor polyPs in these compartments. We propose that CHAD domains may be used to engineer the properties of polyP-metabolizing enzymes and to specifically localize polyP stores in eukaryotic cells and tissues. Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules.,Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi:, 10.26508/lsa.201900385. Print 2019 Jun. PMID:31133615 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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