6qxf

Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system

Loading... Status: waiting for Mol* initialization Structural highlights 6qxf is a 22 chain structure with sequence from Atcc 19258 and Escherichia coli bl21(de3). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA Gene: csn2 (ATCC 19258), cas1 (ATCC 19258), cas2 (ATCC 19258) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed CRISPR and associated Cas proteins function as an adaptive immune system in prokaryotes to combat bacteriophage infection. During the immunization step, new spacers are acquired by the CRISPR machinery, but the molecular mechanism of spacer capture remains enigmatic. We show that the Cas9, Cas1, Cas2, and Csn2 proteins of a Streptococcus thermophilus type II-A CRISPR-Cas system form a complex and provide cryoelectron microscopy (cryo-EM) structures of three different assemblies. The predominant form, with the stoichiometry Cas18-Cas24-Csn28, referred to as monomer, contains approximately 30 bp duplex DNA bound along a central channel. A minor species, termed a dimer, comprises two monomers that sandwich a further eight Cas1 and four Cas2 subunits and contains two DNA approximately 30-bp duplexes within the channel. A filamentous form also comprises Cas18-Cas24-Csn28 units (typically 2-6) but with a different Cas1-Cas2 interface between them and a continuous DNA duplex running along a central channel. Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.,Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB Mol Cell. 2019 May 6. pii: S1097-2765(19)30312-0. doi:, 10.1016/j.molcel.2019.04.020. PMID:31080012 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease 3D structures

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