6rbd

State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles

Loading... Status: waiting for Mol* initialization Structural highlights 6rbd is a 10 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.47Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS25A_YEAST Publication Abstract from PubMed Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps. Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.,Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B Nat Commun. 2019 Jun 21;10(1):2754. doi: 10.1038/s41467-019-10678-z. PMID:31227701 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Adenosine dimethyltransferase 3D structures Ribosome 3D structures Ribosome biogenesis protein 3D structures Serine/threonine protein kinase 3D structures

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