6rl0

Recombinant Pseudomonas stutzeri nitrous oxide reductase, form I

spinqualitylabelsall models 6rl0, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6rl0 is a 4 chain structure with sequence from Pseudomonas stutzeri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.78Å Ligands: B3P, CA, CL, CUA, CUK, FMT, K, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NOSZ_STUST Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. Publication Abstract from PubMed The multicopper enzyme nitrous oxide reductase reduces the greenhouse gas N2O to uncritical N2 as the final step of bacterial denitrification. Its two metal centers require an elaborate assembly machinery that so far has precluded heterologous production as a prerequisite for bioremediatory applications in agriculture and wastewater treatment. Here, we report on the production of active holoenzyme in Escherichia coli using a two-plasmid system to produce the entire biosynthetic machinery as well as the structural gene for the enzyme. Using this recombinant system to probe the role of individual maturation factors, we find that the ABC transporter NosFY and the accessory NosD protein are essential for the formation of the [4Cu:2S] site CuZ, but not the electron transfer site CuA Depending on source organism, the heterologous host E. coli can, in some cases, compensate for the lack of the Cu chaperone NosL, while in others this protein is strictly required, underlining the case for designing a recombinant system to be entirely self-contained. Functional assembly of nitrous oxide reductase provides insights into copper site maturation.,Zhang L, Wust A, Prasser B, Muller C, Einsle O Proc Natl Acad Sci U S A. 2019 Jun 12. pii: 1903819116. doi:, 10.1073/pnas.1903819116. PMID:31189605 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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