6ryo

Bacterial membrane enzyme structure by the in meso method at 1.9 A resolution

spinqualitylabelsall models 6ryo, resolution 1.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ryo is a 2 chain structure with sequence from Staphylococcus aureus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.924Å Ligands: 5BV, 7PE, ALO, GOL, IIL, MLE, OLC, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LSPA_STAAR This protein specifically catalyzes the removal of signal peptides from prolipoproteins. Publication Abstract from PubMed Antimicrobial resistance is a major global threat that calls for new antibiotics. Globomycin and myxovirescin are two natural antibiotics that target the lipoprotein-processing enzyme, LspA, thereby compromising the integrity of the bacterial cell envelope. As part of a project aimed at understanding their mechanism of action and for drug development, we provide high-resolution crystal structures of the enzyme from the human pathogen methicillin-resistant Staphylococcus aureus (MRSA) complexed with globomycin and with myxovirescin. Our results reveal an instance of convergent evolution. The two antibiotics possess different molecular structures. Yet, they appear to inhibit identically as non-cleavable tetrahedral intermediate analogs. Remarkably, the two antibiotics superpose along nineteen contiguous atoms that interact similarly with LspA. This 19-atom motif recapitulates a part of the substrate lipoprotein in its proposed binding mode. Incorporating this motif into a scaffold with suitable pharmacokinetic properties should enable the development of effective antibiotics with built-in resistance hardiness. Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin.,Olatunji S, Yu X, Bailey J, Huang CY, Zapotoczna M, Bowen K, Remskar M, Muller R, Scanlan EM, Geoghegan JA, Olieric V, Caffrey M Nat Commun. 2020 Jan 9;11(1):140. doi: 10.1038/s41467-019-13724-y. PMID:31919415 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA