6s0y

Nanobody targeting influenza A matrix protein 2 ectodomain (M2e)

spinqualitylabelsall models 6s0y, resolution 1.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6s0y is a 4 chain structure with sequence from Influenza A virus (A/Jeju/2279/2007(H1N1)) and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.81Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2VLW8_9INFA Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry.[RuleBase:RU361247] Publication Abstract from PubMed Lower respiratory tract infections, such as infections caused by influenza A viruses, are a constant threat for public health. Antivirals are indispensable to control disease caused by epidemic as well as pandemic influenza A. We developed a novel anti-influenza A virus approach based on an engineered single-domain antibody (VHH) construct that can selectively recruit innate immune cells to the sites of virus replication. This protective construct comprises two VHHs. One VHH binds with nanomolar affinity to the conserved influenza A matrix protein 2 (M2) ectodomain (M2e). Co-crystal structure analysis revealed that the complementarity determining regions 2 and 3 of this VHH embrace M2e. The second selected VHH specifically binds to the mouse Fcgamma Receptor IV (FcgammaRIV) and was genetically fused to the M2e-specific VHH, which resulted in a bi-specific VHH-based construct that could be efficiently expressed in Pichia pastoris. In the presence of M2 expressing or influenza A virus-infected target cells, this single domain antibody construct selectively activated the mouse FcgammaRIV. Moreover, intranasal delivery of this bispecific FcgammaRIV-engaging VHH construct protected wild type but not FcgammaRIV (-/-) mice against challenge with an H3N2 influenza virus. These results provide proof of concept that VHHs directed against a surface exposed viral antigen can be readily armed with effector functions that trigger protective antiviral activity beyond direct virus neutralization. Selective Engagement of FcgammaRIV by a M2e-Specific Single Domain Antibody Construct Protects Against Influenza A Virus Infection.,De Vlieger D, Hoffmann K, Van Molle I, Nerinckx W, Van Hoecke L, Ballegeer M, Creytens S, Remaut H, Hengel H, Schepens B, Saelens X Front Immunol. 2019 Dec 12;10:2920. doi: 10.3389/fimmu.2019.02920. eCollection, 2019. PMID:31921179 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA