6s1t

Structure of beta-fructofuranosidase from Schwanniomyces occidentalis complexed with sucrose

spinqualitylabelsall models 6s1t, resolution 2.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6s1t is a 2 chain structure with sequence from Schwanniomyces occidentalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.09Å Ligands: BMA, EDO, FRU, GLC, NAG, PRD_900003, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E5D0X5_SCHOC Publication Abstract from PubMed The beta-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by [Formula: see text] 30% and 48%, respectively. Moreover, it produced neokestose, which represented [Formula: see text] 27% of total FOS, and yielded 31.8 g l(-1) blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability. New insights into the molecular mechanism behind mannitol and erythritol fructosylation by beta-fructofuranosidase from Schwanniomyces occidentalis.,Rodrigo-Frutos D, Jimenez-Ortega E, Piedrabuena D, Ramirez-Escudero M, Miguez N, Plou FJ, Sanz-Aparicio J, Fernandez-Lobato M Sci Rep. 2021 Mar 30;11(1):7158. doi: 10.1038/s41598-021-86568-6. PMID:33785821 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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