6s3s

Structure of the FliPQR complex from the flagellar type 3 secretion system of Vibrio mimicus.

spinqualitylabelsall models 6s3s, resolution 4.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6s3s is a 10 chain structure with sequence from Vibrio mimicus CAIM 602. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2J9UXT5_VIBMI Plays a role in the flagellum-specific transport system.[RuleBase:RU362069] Publication Abstract from PubMed Protein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins (FliPQR in flagella, SctRST in virulence systems). A fourth essential T3SS protein (FlhB/SctU) functions to "switch" secretion substrate specificity once the growing hook/needle reach their determined length. Here, we present the cryo-electron microscopy structure of an export gate containing the switch protein from a Vibrio flagellar system at 3.2 A resolution. The structure reveals that FlhB/SctU extends the helical export gate with its four predicted transmembrane helices wrapped around FliPQR/SctRST. The unusual topology of the FlhB/SctU helices creates a loop wrapped around the bottom of the closed export gate. Structure-informed mutagenesis suggests that this loop is critical in gating secretion and we propose that a series of conformational changes in the T3SS trigger opening of the gate through interactions between FlhB/SctU and FliPQR/SctRST. The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion.,Kuhlen L, Johnson S, Zeitler A, Baurle S, Deme JC, Caesar JJE, Debo R, Fisher J, Wagner S, Lea SM Nat Commun. 2020 Mar 10;11(1):1296. doi: 10.1038/s41467-020-15071-9. PMID:32157081 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Flagellar protein 3D structures

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