6sgb

mt-SSU assemblosome of Trypanosoma brucei

Loading... Status: waiting for Mol* initialization Structural highlights 6sgb is a 10 chain structure with sequence from Trypanosoma brucei brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.3Å Ligands: GTP, MG, PM8, PO4, SAH, UBD, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q383D1_TRYB2 Publication Abstract from PubMed Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in Trypanosoma brucei involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria. Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.,Saurer M, Ramrath DJF, Niemann M, Calderaro S, Prange C, Mattei S, Scaiola A, Leitner A, Bieri P, Horn EK, Leibundgut M, Boehringer D, Schneider A, Ban N Science. 2019 Sep 13;365(6458):1144-1149. doi: 10.1126/science.aaw5570. PMID:31515389 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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