6slt

Flavin-dependent tryptophan 6-halogenase Thal in complex with tryptophan and FAD

spinqualitylabelsall models 6slt, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6slt is a 2 chain structure with sequence from Streptomyces albogriseolus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: AMP, FAD, GOL, PO4, TRP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A1E280_STRAO Publication Abstract from PubMed Flavin-dependent halogenases require FADH2 , O2 and halide salts to halogenate their substrates. We describe crystal structures of the tryptophan 6-halogenase Thal in complex with FAD or with both tryptophan and FAD. If tryptophan and FAD were soaked simultaneously both ligands showed impaired binding and in some cases only the AMP or the adenosine moiety of FAD was resolved, suggesting that tryptophan binding mainly increases mobility of the FMN moiety. This confirms a negative cooperativity between substrate and cofactor binding previously described for other tryptophan halogenases. Binding of substrate to tryptophan halogenases reduces the affinity for the oxidized cofactor FAD presumably to facilitate regeneration of FADH2 by flavin reductases. This article is protected by copyright. All rights reserved. Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.,Moritzer AC, Niemann HH Protein Sci. 2019 Oct 7. doi: 10.1002/pro.3739. PMID:31589794 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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