6smd

PlMCAT:AntF (holo): type II PKS acyl-carrier protein in complex with its malonyl-transacylase

spinqualitylabelsall models 6smd, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6smd is a 3 chain structure with sequence from Photorhabdus luminescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.3Å Ligands: 4HH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2S8QL96_PHOLU Publication Abstract from PubMed Type II polyketide synthases (PKSs) are multi-enzyme complexes that produce secondary metabolites of medical relevance. Chemical backbones of such polyketides are produced by minimal PKS systems that consist of a malonyl transacylase, an acyl carrier protein and an alpha/beta heterodimeric ketosynthase. Here, we present X-ray structures of all ternary complexes that constitute the minimal PKS system for anthraquinone biosynthesis in Photorhabdus luminescens. In addition, we characterize this invariable core using molecular simulations, mutagenesis experiments and functional assays. We show that malonylation of the acyl carrier protein is accompanied by major structural rearrangements in the transacylase. Principles of an ongoing chain elongation are derived from the ternary complex with a hexaketide covalently linking the heterodimeric ketosynthase with the acyl carrier protein. Our results for the minimal PKS system provide mechanistic understanding of PKSs and a fundamental basis for engineering PKS pathways for future applications. Structural snapshots of the minimal PKS system responsible for octaketide biosynthesis.,Brauer A, Zhou Q, Grammbitter GLC, Schmalhofer M, Ruhl M, Kaila VRI, Bode HB, Groll M Nat Chem. 2020 Aug;12(8):755-763. doi: 10.1038/s41557-020-0491-7. Epub 2020 Jul, 6. PMID:32632186 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Acyl carrier protein 3D structures

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