6sp2

CryoEM structure of SERINC from Drosophila melanogaster

Loading... Status: waiting for Mol* initialization Structural highlights 6sp2 is a 6 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.33Å Ligands: CDL, LMN, P5S Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9U6P4_DROME Publication Abstract from PubMed The human integral membrane protein SERINC5 potently restricts HIV-1 infectivity and sensitizes the virus to antibody-mediated neutralization. Here, using cryo-EM, we determine the structures of human SERINC5 and its orthologue from Drosophila melanogaster at subnanometer and near-atomic resolution, respectively. The structures reveal a novel fold comprised of ten transmembrane helices organized into two subdomains and bisected by a long diagonal helix. A lipid binding groove and clusters of conserved residues highlight potential functional sites. A structure-based mutagenesis scan identified surface-exposed regions and the interface between the subdomains of SERINC5 as critical for HIV-1-restriction activity. The same regions are also important for viral sensitization to neutralizing antibodies, directly linking the antiviral activity of SERINC5 with remodeling of the HIV-1 envelope glycoprotein. A bipartite structural organization defines the SERINC family of HIV-1 restriction factors.,Pye VE, Rosa A, Bertelli C, Struwe WB, Maslen SL, Corey R, Liko I, Hassall M, Mattiuzzo G, Ballandras-Colas A, Nans A, Takeuchi Y, Stansfeld PJ, Skehel JM, Robinson CV, Pizzato M, Cherepanov P Nat Struct Mol Biol. 2020 Jan;27(1):78-83. doi: 10.1038/s41594-019-0357-0. Epub, 2020 Jan 6. PMID:31907454 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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