6syf

Human Ubc9 with covalent isopeptide ligand

spinqualitylabelsall models 6syf, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6syf is a 12 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: ACE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Enzymes are powerful tools for protein labelling due to their specificity and mild reaction conditions. Many protocols, however, are restricted to modifications at protein termini, rely on non-peptidic metabolites or require large recognition domains. Here we report a chemoenzymatic method, which we call lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at internal lysine residues. LACE relies on a minimal genetically encoded tag (four residues) recognized by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters. Together, this approach obviates the need for E1 and E3 enzymes, enabling isopeptide formation with just Ubc9 in a programmable manner. We demonstrate the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling in combination with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins. Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins.,Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW Nat Chem. 2020 Sep 14. pii: 10.1038/s41557-020-0528-y. doi:, 10.1038/s41557-020-0528-y. PMID:32929246 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also SUMO conjugating enzyme Ubc9

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