6t75

Bacteroides salyersiae GH164 beta-mannosidase 2-deoxy-2-fluoro-beta-D-mannosyl enzyme intermediate

spinqualitylabelsall models 6t75, resolution 2.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6t75 is a 6 chain structure with sequence from Bacteroides salyersiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.55Å Ligands: CL, MAF Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I9SUA3_9BACE Publication Abstract from PubMed Recent work exploring protein sequence space has revealed a new glycoside hydrolase (GH) family (GH164) of putative mannosidases. GH164 genes are present in several commensal bacteria, implicating these genes in the degradation of dietary glycans. However, little is known about the structure, mechanism of action and substrate specificity of these enzymes. Herein we report the biochemical characterization and crystal structures of the founding member of this family (Bs164) from the human gut symbiont Bacteroides salyersiae. Previous reports of this enzyme indicated that it has alpha-mannosidase activity, however we conclusively show that it cleaves only beta-mannose linkages. Using NMR spectroscopy, detailed enzyme kinetics of wild-type and mutant Bs164, and multi-angle light scattering we found that it is a trimeric retaining beta-mannosidase, that is susceptible to several known mannosidase inhibitors. X-ray crystallography revealed the structure of Bs164 - the first known structure of a GH164 - at 1.91 A resolution. Bs164 is composed of three domains: a (beta/alpha)8 barrel, a trimerization domain and a beta-sandwich domain, representing a previously unobserved structural fold for beta-mannosidases. Structures of Bs164 at 1.80-2.55 A resolution in complex with the inhibitors noeuromycin, mannoimidazole or DNP 2-deoxy-2-fluoro-mannose reveal the residues essential for specificity and catalysis including the catalytic nucleophile (Glu297) and acid/base residue (Glu160). These findings further our knowledge of the mechanisms commensal microbes use for nutrient acquisition. Structure and function of Bs164 beta-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164.,Armstrong Z, Davies GJ J Biol Chem. 2019 Dec 22. pii: RA119.011591. doi: 10.1074/jbc.RA119.011591. PMID:31871050 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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