6tch

Binary complex of 14-3-3 sigma and a high-affinity non-canonical 9-mer peptide binder

spinqualitylabelsall models 6tch, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6tch is a 2 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.801Å Ligands: B3S, BAL, CL, DLY, KCJ, MG, NH2, NVA, PPN, SEP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function 1433S_HUMAN Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression. Publication Abstract from PubMed High-diversity genetically-encoded combinatorial libraries (10(8)-10(13) members) are a rich source of peptide-based binding molecules, identified by affinity selection. Synthetic libraries can access broader chemical space, but typically examine only ~ 10(6) compounds by screening. Here we show that in-solution affinity selection can be interfaced with nano-liquid chromatography-tandem mass spectrometry peptide sequencing to identify binders from fully randomized synthetic libraries of 10(8) members-a 100-fold gain in diversity over standard practice. To validate this approach, we show that binders to a monoclonal antibody are identified in proportion to library diversity, as diversity is increased from 10(6)-10(8). These results are then applied to the discovery of p53-like binders to MDM2, and to a family of 3-19 nM-affinity, alpha/beta-peptide-based binders to 14-3-3. An X-ray structure of one of these binders in complex with 14-3-3sigma is determined, illustrating the role of beta-amino acids in facilitating a key binding contact. Ultra-large chemical libraries for the discovery of high-affinity peptide binders.,Quartararo AJ, Gates ZP, Somsen BA, Hartrampf N, Ye X, Shimada A, Kajihara Y, Ottmann C, Pentelute BL Nat Commun. 2020 Jun 23;11(1):3183. doi: 10.1038/s41467-020-16920-3. PMID:32576815 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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