6te4

Structural insights into Pseudomonas aeruginosa Type six secretion system exported effector 8: Tse8 in complex with a peptide

spinqualitylabelsall models 6te4, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6te4 is a 3 chain structure with sequence from Escherichia coli BL21(DE3) and Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.29Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9HWL8_PSEAE Publication Abstract from PubMed Recent reports indicate that the Type six secretion system exported effector 8 (Tse8) is a cytoactive effector secreted by the Type VI secretion system (T6SS) of the human pathogen Pseudomonas aeruginosa. The T6SS is a nanomachine that assembles inside of the bacteria and injects effectors/toxins into target cells, providing a fitness advantage over competing bacteria and facilitating host colonisation. Here we present the first crystal structure of Tse8 revealing that it conserves the architecture of the catalytic triad Lys84-transSer162-Ser186 that characterises members of the Amidase Signature superfamily. Furthermore, using binding affinity experiments, we show that the interaction of phenylmethylsulfonyl fluoride (PMSF) to Tse8 is dependent on the putative catalytic residue Ser186, providing support for its nucleophilic reactivity. This work thus demonstrates that Tse8 belongs to the Amidase Signature (AS) superfamily. Furthermore, it highlights Tse8 similarity to two family members: the Stenotrophomonas maltophilia Peptide Amidase and the Glutamyl-tRNA(Gln) amidotransferase subunit A from Staphylococcus aureus. Structural insights into Pseudomonas aeruginosaType six secretion system exported effector 8.,Gonzalez-Magana A, Sainz-Polo MA, Pretre G, Capuni R, Lucas M, Altuna J, Montanchez I, Fucini P, Albesa-Jove D J Struct Biol. 2020 Oct 20;212(3):107651. doi: 10.1016/j.jsb.2020.107651. PMID:33096229 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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