6teh

Baseplate of native GTA particle computed with C3 symmetry

spinqualitylabelsall models 6teh, resolution 3.99Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6teh is a 4 chain structure with sequence from Rhodobacter capsulatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.99Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A9U0_RHOCA Publication Abstract from PubMed Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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