6tsm

Marasmius oreades agglutinin (MOA) in complex with the truncated PVVRAHS synthetic substrate

spinqualitylabelsall models 6tsm, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6tsm is a 2 chain structure with sequence from Marasmius oreades and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4Å Ligands: CA, EDO, FUC, GAL, GLA, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8X123_9AGAR Publication Abstract from PubMed The Marasmius oreades agglutinin (MOA) is the holotype of an emerging family of fungal chimerolectins and an active Ca(2+)/Mn(2+)-dependent protease, which exhibits a unique papain-like fold with special active site features. Here we investigated the functional significance of the structural elements differentiating MOA from other papain-like cysteine proteases. X-ray crystal structures of MOA co-crystallized with two synthetic substrates reveal cleaved peptides bound to the catalytic site, corresponding to the final products of the proteolytic reaction. Anomalous diffraction data on crystals grown in the presence of calcium and manganese, cadmium or zinc resolve the calcium/manganese preference of MOA and elucidate the inhibitory roles of zinc and cadmium towards papain-like cysteine proteases in general. The reported structures, together with activity data of MOA active site variants, point to a conservation of the general proteolysis mechanism established for papain. Ultimately, the findings suggest that papain and the papain-like domain of MOA are the product of convergent evolution. Crystal structure of MOA in complex with a peptide fragment: A protease caught in flagranti.,Manna D, Cordara G, Krengel U Curr Res Struct Biol. 2020 Apr 22;2:56-67. doi: 10.1016/j.crstbi.2020.04.003., eCollection 2020. PMID:34235469 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Agglutinin 3D structures

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