6u18

Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin

spinqualitylabelsall models 6u18, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6u18 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: CTY, FLC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9EVJ6_ECOLX Publication Abstract from PubMed Clarithromycin is an improved semisynthetic analogue of the naturally occurring macrolide, erythromycin. The subtle modification of a methyl group on the C-6 hydroxyl group endows the molecule with improved acid stability and results in a clinically useful antibiotic. Here, we show that the effector specificity of the biosensor protein, MphR, can be evolved to selectively recognize clarithromycin and therefore report on the production of this molecule in vivo. In addition, a crystal structure of the evolved variant reveals the molecular basis for selectivity and provides a guide for the evolution of a new metabolic function using this biosensor. Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics.,Li Y, Reed M, Wright HT, Cropp TA, Williams GJ ACS Synth Biol. 2021 Sep 21. doi: 10.1021/acssynbio.1c00151. PMID:34546703 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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