6u7l
2.75 Angstrom Crystal Structure of Galactarate Dehydratase from Escherichia coli.
Structural highlights
FunctionGARD_ECOLI Catalyzes the dehydration of galactarate to form 5-dehydro-4-deoxy-D-glucarate.[HAMAP-Rule:MF_02031][1] Publication Abstract from PubMedGalactarate dehydratase (GarD) is the first enzyme in the galactarate/glucarate pathway and catalyzes the dehydration of galactarate to 3-keto-5-dehydroxygalactarate. This protein is known to increase colonization fitness of intestinal pathogens in antibiotic-treated mice and to promote bacterial survival during stress. The galactarate/glucarate pathway is widespread in bacteria, but not in humans, and thus could be a target to develop new inhibitors for use in combination therapy to combat antibiotic resistance. The structure of almost all the enzymes of the galactarate/glucarate pathway were solved previously, except for GarD, for which only the structure of the N-terminal domain was determined previously. Herein, we report the first crystal structure of full-length GarD solved using a seleno-methoionine derivative revealing a new protein fold. The protein consists of three domains, each presenting a novel twist as compared to their distant homologs. GarD in the crystal structure forms dimers and each monomer consists of three domains. The N-terminal domain is comprised of a beta-clip fold, connected to the second domain by a long unstructured linker. The second domain serves as a dimerization interface between two monomers. The C-terminal domain forms an unusual variant of a Rossmann fold with a crossover and is built around a seven-stranded parallel beta-sheet supported by nine alpha-helices. A metal binding site in the C-terminal domain is occupied by Ca(2+) . The activity of GarD was corroborated by the production of 5-keto-4-deoxy-D-glucarate under reducing conditions and in the presence of iron. Thus, GarD is an unusual enolase with a novel protein fold never previously seen in this class of enzymes. Structure of galactarate dehydratase, a new fold in an enolase involved in bacterial fitness after antibiotic treatment.,Rosas-Lemus M, Minasov G, Shuvalova L, Wawrzak Z, Kiryukhina O, Mih N, Jaroszewski L, Palsson B, Godzik A, Satchell KJF Protein Sci. 2020 Mar;29(3):711-722. doi: 10.1002/pro.3796. Epub 2019 Dec 17. PMID:31811683[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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