6uo1

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution

spinqualitylabelsall models 6uo1, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6uo1 is a 20 chain structure with sequence from Escherichia virus T4 and Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.95Å Ligands: 0TD, 2MA, 2MG, 2MU, 4OC, 4SU, 5MC, 5MU, 7MG, M2G, MA6, MG, MIA, OMG, PSU, UR3, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS10_THET8 Part of the top of the 30S subunit head.[HAMAP-Rule:MF_00508] Publication Abstract from PubMed Chemical modifications of RNAs have long been established as key modulators of nonprotein-coding RNA structure and function in cells. There is a growing appreciation that messenger RNA (mRNA) sequences responsible for directing protein synthesis can also be posttranscriptionally modified. The enzymatic incorporation of mRNA modifications has many potential outcomes, including changing mRNA stability, protein recruitment, and translation. We tested how one of the most common modifications present in mRNA coding regions, pseudouridine (Psi), impacts protein synthesis using a fully reconstituted bacterial translation system and human cells. Our work reveals that replacing a single uridine nucleotide with Psi in an mRNA codon impedes amino acid addition and EF-Tu GTPase activation. A crystal structure of the Thermus thermophilus 70S ribosome with a tRNA(Phe) bound to a PsiUU codon in the A site supports these findings. We also find that the presence of Psi can promote the low-level synthesis of multiple peptide products from a single mRNA sequence in the reconstituted translation system as well as human cells, and increases the rate of near-cognate Val-tRNA(Val) reacting on a PsiUU codon. The vast majority of Psi moieties in mRNAs are found in coding regions, and our study suggests that one consequence of the ribosome encountering Psi can be to modestly alter both translation speed and mRNA decoding. Pseudouridinylation of mRNA coding sequences alters translation.,Eyler DE, Franco MK, Batool Z, Wu MZ, Dubuke ML, Dobosz-Bartoszek M, Jones JD, Polikanov YS, Roy B, Koutmou KS Proc Natl Acad Sci U S A. 2019 Nov 12;116(46):23068-23074. doi:, 10.1073/pnas.1821754116. Epub 2019 Oct 31. PMID:31672910 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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