6vmi

Structure of the human mitochondrial ribosome-EF-G1 complex (ClassIII)

spinqualitylabelsall models 6vmi, resolution 2.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6vmi is a 12 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.96Å Ligands: GCP, GDP, MG, P5P, Y5P, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RT10_HUMAN Publication Abstract from PubMed The mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we present the 2.68-3.96 A cryo-EM structures of the human 55S mitoribosome in complex with the human mitochondrial elongation factor G1 (EF-G1(mt)) in three distinct conformational states, including an intermediate state and a post-translocational state. These structures reveal the role of several mitochondria-specific (mito-specific) mitoribosomal proteins (MRPs) and a mito-specific segment of EF-G1(mt) in mitochondrial tRNA (tRNA(mt)) translocation. In particular, the mito-specific C-terminal extension in EF-G1(mt) is directly involved in translocation of the acceptor arm of the A-site tRNA(mt). In addition to the ratchet-like and independent head-swiveling motions exhibited by the small mitoribosomal subunit, we discover significant conformational changes in MRP mL45 at the nascent polypeptide-exit site within the large mitoribosomal subunit that could be critical for tethering of the elongating mitoribosome onto the inner-mitochondrial membrane. Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation.,Koripella RK, Sharma MR, Bhargava K, Datta PP, Kaushal PS, Keshavan P, Spremulli LL, Banavali NK, Agrawal RK Nat Commun. 2020 Jul 31;11(1):3830. doi: 10.1038/s41467-020-17715-2. PMID:32737313 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Elongation factor 3D structures Ribosome 3D structures

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