6wk5

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters

spinqualitylabelsall models 6wk5, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6wk5 is a 4 chain structure with sequence from Clostridiales bacterium oral taxon 876 str. F0540 and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function U2EQ00_9FIRM Publication Abstract from PubMed By providing broad resistance to environmental biocides, transporters from the small multidrug resistance (SMR) family drive the spread of multidrug resistance cassettes among bacterial populations. A fundamental understanding of substrate selectivity by SMR transporters is needed to identify the types of selective pressures that contribute to this process. Using solid-supported membrane electrophysiology, we find that promiscuous transport of hydrophobic substituted cations is a general feature of SMR transporters. To understand the molecular basis for promiscuity, we solved X-ray crystal structures of a SMR transporter Gdx-Clo in complex with substrates to a maximum resolution of 2.3 A. These structures confirm the family's extremely rare dual topology architecture and reveal a cleft between two helices that provides accommodation in the membrane for the hydrophobic substituents of transported drug-like cations. The structural basis of promiscuity in small multidrug resistance transporters.,Kermani AA, Macdonald CB, Burata OE, Ben Koff B, Koide A, Denbaum E, Koide S, Stockbridge RB Nat Commun. 2020 Nov 27;11(1):6064. doi: 10.1038/s41467-020-19820-8. PMID:33247110 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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