6wk7

Crystal Structure Analysis of a poly(thymine) DNA duplex

spinqualitylabelsall models 6wk7, resolution 2.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6wk7 is a 2 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.423Å Ligands: AX2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The diversity of DNA duplex structures is limited by a binary pair of hydrogen-bonded motifs. Here we show that poly(thymine) self-associates into antiparallel, right-handed duplexes in the presence of melamine, a small molecule that presents a triplicate set of the hydrogen-bonding face of adenine. X-ray crystallography shows that in the complex two poly(thymine) strands wrap around a helical column of melamine, which hydrogen bonds to thymine residues on two of its three faces. The mechanical strength of the thymine-melamine-thymine triplet surpasses that of adenine-thymine base pairs, which enables a sensitive detection of melamine at 3 pM. The poly(thymine)-melamine duplex is orthogonal to native DNA base pairing and can undergo strand displacement without the need for overhangs. Its incorporation into two-dimensional grids and hybrid DNA-small-molecule polymers highlights the poly(thymine)-melamine duplex as an additional tool for DNA nanotechnology. A poly(thymine)-melamine duplex for the assembly of DNA nanomaterials.,Li Q, Zhao J, Liu L, Jonchhe S, Rizzuto FJ, Mandal S, He H, Wei S, Sleiman HF, Mao H, Mao C Nat Mater. 2020 Jul 13. pii: 10.1038/s41563-020-0728-2. doi:, 10.1038/s41563-020-0728-2. PMID:32661383 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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