6wo1

Hybrid acetohydroxyacid synthase complex structure with Cryptococcus neoformans AHAS catalytic subunit and Saccharomyces cerevisiae AHAS regulatory subunit

spinqualitylabelsall models 6wo1, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6wo1 is a 2 chain structure with sequence from Cryptococcus neoformans and Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.3Å Ligands: 8GF, DPO, FAD, MG, VAL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q96VZ6_CRYNE Publication Abstract from PubMed Acetohydroxyacid synthase (AHAS), also known as acetolactate synthase, is a FAD and ThDP-Mg(2+)-dependent enzyme that catalyses the first step in the biosynthesis of the branched chain amino acids (BCAAs)(1). It is the target for over 50 commercial herbicides(2). AHAS requires both catalytic and regulatory subunits for maximal activity and functionality. Herein, our structures of the hexadecameric Saccharomyces cerevisiae and dodecameric Arabidopsis thaliana AHAS complexes demonstrate that the regulatory subunits form a core upon which the catalytic subunit dimers are attached, adopting the shape of a "Maltese Cross". The structures show how the catalytic and regulatory subunits communicate with each other to provide a pathway for activation and for feedback inhibition by the BCAAs. We also show that Mycobacterium tuberculosis AHAS adopts a similar structure, thus demonstrating that the overall AHAS architecture is conserved across kingdoms. Structures of fungal and plant acetohydroxyacid synthases.,Lonhienne T, Low YS, Garcia MD, Croll T, Gao Y, Wang Q, Brillault L, Williams CM, Fraser JA, McGeary RP, West NP, Landsberg MJ, Rao Z, Schenk G, Guddat LW Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2514-3. doi:, 10.1038/s41586-020-2514-3. PMID:32640464 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA