6xbo

X-ray crystal structure of the mouse CMP-Sialic acid transporter in complex with 5-methyl CMP

spinqualitylabelsall models 6xbo, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xbo is a 1 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: 2PE, 5MC, OLC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function S35A1_MOUSE Transport CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. Publication Abstract from PubMed Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans. We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019). These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures. Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST. The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes. Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation. Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.,Ahuja S, Cahill J, Hartfield K, Whorton MR PLoS One. 2021 Jun 3;16(6):e0249905. doi: 10.1371/journal.pone.0249905., eCollection 2021. PMID:34081697 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA