6xlp

Structure of the essential inner membrane lipopolysaccharide-PbgA complex

spinqualitylabelsall models 6xlp, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xlp is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: 3PE, KDO, OLB, PG4, SO4, VJ1, VJ4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function YEJM_ECOLI Publication Abstract from PubMed Lipopolysaccharide (LPS) resides in the outer membrane of Gram-negative bacteria where it is responsible for barrier function(1,2). LPS can cause death as a result of septic shock, and its lipid A core is the target of polymyxin antibiotics(3,4). Despite the clinical importance of polymyxins and the emergence of multidrug resistant strains(5), our understanding of the bacterial factors that regulate LPS biogenesis is incomplete. Here we characterize the inner membrane protein PbgA and report that its depletion attenuates the virulence of Escherichia coli by reducing levels of LPS and outer membrane integrity. In contrast to previous claims that PbgA functions as a cardiolipin transporter(6-9), our structural analyses and physiological studies identify a lipid A-binding motif along the periplasmic leaflet of the inner membrane. Synthetic PbgA-derived peptides selectively bind to LPS in vitro and inhibit the growth of diverse Gram-negative bacteria, including polymyxin-resistant strains. Proteomic, genetic and pharmacological experiments uncover a model in which direct periplasmic sensing of LPS by PbgA coordinates the biosynthesis of lipid A by regulating the stability of LpxC, a key cytoplasmic biosynthetic enzyme(10-12). In summary, we find that PbgA has an unexpected but essential role in the regulation of LPS biogenesis, presents a new structural basis for the selective recognition of lipids, and provides opportunities for future antibiotic discovery. Structure of the essential inner membrane lipopolysaccharide-PbgA complex.,Clairfeuille T, Buchholz KR, Li Q, Verschueren E, Liu P, Sangaraju D, Park S, Noland CL, Storek KM, Nickerson NN, Martin L, Dela Vega T, Miu A, Reeder J, Ruiz-Gonzalez M, Swem D, Han G, DePonte DP, Hunter MS, Gati C, Shahidi-Latham S, Xu M, Skelton N, Sellers BD, Skippington E, Sandoval W, Hanan EJ, Payandeh J, Rutherford ST Nature. 2020 Aug;584(7821):479-483. doi: 10.1038/s41586-020-2597-x. Epub 2020 Aug, 12. PMID:32788728 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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