6xur

RNA dodecamer with a 6-hydrazino-2-aminopurine modified base

spinqualitylabelsall models 6xur, resolution 1.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xur is a 1 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.1Å Ligands: MG, O2Z Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Temporal information about cellular RNA populations is essential to understand the functional roles of RNA. We have developed hydrazine/NH 4 Cl/OsO 4 -based conversion of 6-thioguanosine (6sG)- into A'-containing RNA, where A' constitutes a 6-hydrazino purine derivative. The A' nucleoside retains the Watson-Crick base pair mode as shown by crystal structure analysis of a short palindromic duplex and thermodynamic analysis of UV-melting profiles. We further demonstrate that A' is efficiently decoded as adenosine in primer extension assays and in RNA sequencing experiments. Because 6sG is applicable to metabolic labeling of freshly synthesized RNA and because the novel conversion chemistry is fully compatible with the conversion of the frequently applied metabolic label 4-thiouridine (4sU) into C, the combination of both modified nucleosides in dual labeling set-ups enables high accuracy measurements of RNA decay. Our novel approach that we name TUC-seq DUAL uses the two modified nucleotides in subsequent pulses and their simultaneous detection. Thus, synthesis and decay of mRNA can be clearly distinguished based on the differential presence of G-to-A and U-to-C mutations. This enables mRNA life-time evaluation with unprecedented precision. Thioguanosine conversion enables mRNA life-time evaluation by RNA sequencing via double metabolic labeling.,Gasser C, Delazer I, Neuner E, Pascher K, Brillet K, Klotz S, Trixl L, Himmelstoss M, Ennifar E, Rieder D, Lusser A, Micura R Angew Chem Int Ed Engl. 2020 Jan 30. doi: 10.1002/anie.201916272. PMID:31999864 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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