6xye

Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain

spinqualitylabelsall models 6xye, resolution 4.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xye is a 6 chain structure with sequence from Morbillivirus canis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0R5ZPI3_9MONO Publication Abstract from PubMed Measles virus (MeV) and canine distemper virus (CDV), two members of the Morbillivirus genus, are still causing important global diseases of humans and animals, respectively. To enter target cells, morbilliviruses rely on an envelope-anchored machinery, which is composed of two interacting glycoproteins: a tetrameric receptor binding (H) protein and a trimeric fusion (F) protein. To execute membrane fusion, the F protein initially adopts a metastable, prefusion state that refolds into a highly stable postfusion conformation as the result of a finely coordinated activation process mediated by the H protein. Here, we employed cryo-electron microscopy (cryo-EM) and single particle reconstruction to elucidate the structure of the prefusion state of the CDV F protein ectodomain (solF) at 4.3 A resolution. Stabilization of the prefusion solF trimer was achieved by fusing the GCNt trimerization sequence at the C-terminal protein region, and expressing and purifying the recombinant protein in the presence of a morbilliviral fusion inhibitor class compound. The three-dimensional cryo-EM map of prefusion CDV solF in complex with the inhibitor clearly shows density for the ligand at the protein binding site suggesting common mechanisms of membrane fusion activation and inhibition employed by different morbillivirus members. Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain.,Kalbermatter D, Shrestha N, Gall FM, Wyss M, Riedl R, Plattet P, Fotiadis D J Struct Biol X. 2020 Feb 29;4:100021. doi: 10.1016/j.yjsbx.2020.100021., eCollection 2020. PMID:32647825 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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