6y0h

High resolution structure of GH11 xylanase from Nectria haematococca

spinqualitylabelsall models 6y0h, resolution 1.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6y0h is a 1 chain structure with sequence from Fusarium vanettenii 77-13-4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C7YSL3_FUSV7 Publication Abstract from PubMed Enzymatic degradation of vegetal biomass offers versatile procedures to improve the production of alternative fuels and other biomass-based products. Here we present the three-dimensional structure of a xylanase from Nectria haematococca (NhGH11) at 1.0 A resolution and its functional properties. The atomic resolution structure provides details and insights about the complex hydrogen bonding network of the active site region and allowed a detailed comparison with homologous structures. Complementary biochemical studies showed that the xylanase can catalyze the hydrolysis of complex xylan into simple xylose aldopentose subunits of different lengths. NhGH11 can catalyze the efficient breakdown of beechwood xylan, xylan polysaccharide, and wheat arabinoxylan with turnover numbers of 1730.6 +/- 318.1 min(-1), 1648.2 +/- 249.3 min(-1) and 2410.8 +/- 517.5 min(-1) respectively. NhGH11 showed maximum catalytic activity at pH 6.0 and 45 degrees C. The mesophilic character of NhGH11 can be explained by distinct structural features in comparison to thermophilic GH11 enzymes, including the number of hydrogen bonds, side chain interactions and number of buried water molecules. The enzymatic activity of NhGH11 is not very sensitive to metal ions and chemical reagents that are typically present in associated industrial production processes. The data we present highlights the potential of NhGH11 to be applied in industrial biomass degradation processes. High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca.,Andaleeb H, Ullah N, Falke S, Perbandt M, Brognaro H, Betzel C Sci Rep. 2020 Sep 24;10(1):15658. doi: 10.1038/s41598-020-72644-w. PMID:32973265 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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