6yai

Clathrin with bound beta2 appendage of AP2

spinqualitylabelsall models 6yai, resolution 9.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yai is a 14 chain structure with sequence from Homo sapiens and Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 9.2Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I3LGD4_PIG Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.[PIRNR:PIRNR002290] Publication Abstract from PubMed Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell's plasma membrane. Clathrin forms a cage-like, polyhedral outer scaffold around a vesicle, to which cargo-selecting clathrin adaptors are attached. Adaptor protein complex (AP2) is the key adaptor in CME. Crystallography has shown AP2 to adopt a range of conformations. Here, we used cryo-electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane-assembled clathrin-coated vesicles (CCVs). AP2 binds cargo and PtdIns(4,5)P 2 (phosphatidylinositol 4,5-bisphosphate)-containing membranes via multiple interfaces, undergoing conformational rearrangement from its cytosolic state. The binding mode of AP2 beta2 appendage into the clathrin lattice in CCVs and buds implies how the adaptor structurally modulates coat curvature and coat disassembly. Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.,Kovtun O, Dickson VK, Kelly BT, Owen DJ, Briggs JAG Sci Adv. 2020 Jul 22;6(30):eaba8381. doi: 10.1126/sciadv.aba8381. eCollection, 2020 Jul. PMID:32743075 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Adaptin 3D structures Clathrin 3D structures

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