6yge

NADase from Aspergillus fumigatus

spinqualitylabelsall models 6yge, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yge is a 2 chain structure with sequence from Aspfu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, BMA, CA, NAG, FUC, MAN Gene: AFUA_6G14470 (ASPFU) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Nicotinamide adenine dinucleotide (NAD) is a key molecule in cellular bioenergetics and signalling. Various bacterial pathogens release NADase enzymes into the host cell that deplete the host's NAD(+) pool, thereby causing rapid cell death. Here, we report the identification of NADases on the surface of fungi such as the pathogen Aspergillus fumigatus and the saprophyte Neurospora crassa. The enzymes harbour a tuberculosis necrotizing toxin (TNT) domain and are predominately present in pathogenic species. The 1.6 A X-ray structure of the homodimeric A. fumigatus protein reveals unique properties including N-linked glycosylation and a Ca(2+)-binding site whose occupancy regulates activity. The structure in complex with a substrate analogue suggests a catalytic mechanism that is distinct from those of known NADases, ADP-ribosyl cyclases and transferases. We propose that fungal NADases may convey advantages during interaction with the host or competing microorganisms. Discovery of fungal surface NADases predominantly present in pathogenic species.,Stromland O, Kallio JP, Pschibul A, Skoge RH, Harethardottir HM, Sverkeli LJ, Heinekamp T, Kniemeyer O, Migaud M, Makarov MV, Gossmann TI, Brakhage AA, Ziegler M Nat Commun. 2021 Mar 12;12(1):1631. doi: 10.1038/s41467-021-21307-z. PMID:33712585 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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