6yhh

X-ray Structure of Flavobacterium johnsoniae chitobiase (FjGH20)

spinqualitylabelsall models 6yhh, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yhh is a 2 chain structure with sequence from Flavobacterium johnsoniae UW101. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.7Å Ligands: GOL, TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A5FB64_FLAJ1 Publication Abstract from PubMed Chitin is one of the most abundant renewable organic materials found on earth. The chitin utilization locus in Flavobacterium johnsoniae, which encodes necessary proteins for complete enzymatic depolymerization of crystalline chitin, has recently been characterized but no detailed structural information on the enzymes was provided. Here we present protein structures of the F. johnsoniae chitobiase (FjGH20) and chitinase B (FjChiB). FjGH20 is a multi-domain enzyme with a helical domain not before observed in other chitobiases and a domain organization reminiscent of GH84 (beta-N-acetylglucosaminidase) family members. The structure of FjChiB reveals that the protein lacks loops and regions associated with exo-acting activity in other chitinases and instead has a more solvent accessible substrate binding cleft, which is consistent with its endo-chitinase activity. Additionally, small angle X-ray scattering data were collected for the internal 70 kDa region that connects the N- and C-terminal chitinase domains of the unique 158 kDa multi-domain chitinase A (FjChiA). The resulting model of the molecular envelope supports bioinformatic predictions of the region comprising six domains, each with similarities to either Fn3-like or Ig-like domains. Taken together, the results provide insights into chitin utilization by F. johnsoniae and reveal structural diversity in bacterial chitin metabolism. Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae.,Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Branden G, Larsbrink J Sci Rep. 2020 Aug 13;10(1):13775. doi: 10.1038/s41598-020-70749-w. PMID:32792608 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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