6yi5

In-situ structure of the trimeric HEF from influenza C by flexible fitting into a cryo-ET map.

spinqualitylabelsall models 6yi5, resolution 9.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yi5 is a 6 chain structure with sequence from Influenza C virus (C/Johannesburg/1/66). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 9.1Å Ligands: BMA, NAG, NDG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The lipid-enveloped influenza C virus contains a single surface glycoprotein, the haemagglutinin-esterase-fusion (HEF) protein, that mediates receptor binding, receptor destruction, and membrane fusion at the low pH of the endosome. Here we apply electron cryotomography and subtomogram averaging to describe the structural basis for hexagonal lattice formation by HEF on the viral surface. The conformation of the glycoprotein in situ is distinct from the structure of the isolated trimeric ectodomain, showing that a splaying of the membrane distal domains is required to mediate contacts that form the lattice. The splaying of these domains is also coupled to changes in the structure of the stem region which is involved in membrane fusion, thereby linking HEF's membrane fusion conformation with its assembly on the virus surface. The glycoprotein lattice can form independent of other virion components but we show a major role for the matrix layer in particle formation. In situ structure and organization of the influenza C virus surface glycoprotein.,Halldorsson S, Sader K, Turner J, Calder LJ, Rosenthal PB Nat Commun. 2021 Mar 16;12(1):1694. doi: 10.1038/s41467-021-21818-9. PMID:33727554 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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