6yux

Crystal structure of Malus domestica Double Bond Reductase (MdDBR) ternary complex

spinqualitylabelsall models 6yux, resolution 1.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yux is a 1 chain structure with sequence from Malus domestica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.36Å Ligands: EDO, FER, MPD, NA, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In this study we describe the crystal structures of the apoform, the binary and the ternary complexes of a double bond reductase from Malus domestica L. (MdDBR) and explore a range of potential substrates. The overall fold of MdDBR is similar to that of the medium chain reductase/dehydrogenase/zinc-dependent alcohol dehydrogenase-like family. Structural comparison of MdDBR with Arabidopsis thaliana DBR (AtDBR), Nicotiana tabacum DBR (NtDBR) and Rubus idaeus DBR (RiDBR) allowed the identification of key amino acids involved in cofactor and ligands binding and shed light on how these residues may guide the orientation of the substrates. The enzyme kinetic for the substrate trans-4-phenylbuten-2-one has been analyzed, and MdDBR activity towards a variety of substrates was tested. This enzyme has been reported to be involved in the phenylpropanoid pathway where it would catalyze the NADPH-dependent reduction of the alpha, beta-unsaturated double bond of carbonyl metabolites. Our study provides new data towards the identification of MdDBR natural substrate and the biosynthetic pathway where it belongs. Furthermore, the originally proposed involvement in dihydrochalcone biosynthesis in apple must be questioned. The structural and functional characterization of Malus domestica double bond reductase MdDBR provides insights towards the identification of its substrates.,Caliandro R, Polsinelli I, Demitri N, Musiani F, Martens S, Benini S Int J Biol Macromol. 2021 Jan 4;171:89-99. doi: 10.1016/j.ijbiomac.2020.12.190. PMID:33412202 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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