6ywv

The structure of the Atp25 bound assembly intermediate of the mitoribosome from Neurospora crassa

spinqualitylabelsall models 6ywv, resolution 3.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ywv is a 10 chain structure with sequence from Neurospora crassa OR74A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.03Å Ligands: K, MG, NAD, SPM, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q7RZF0_NEUCR Publication Abstract from PubMed Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF(1)) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation. Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.,Itoh Y, Naschberger A, Mortezaei N, Herrmann JM, Amunts A Nat Commun. 2020 Oct 14;11(1):5187. doi: 10.1038/s41467-020-18830-w. PMID:33056988 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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