6yxx

State A of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate

spinqualitylabelsall models 6yxx, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yxx is a 11 chain structure with sequence from Trypanosoma brucei brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.9Å Ligands: ATP, GTP, MG, N, NA, NAD, PM8, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C9ZY77_TRYB9 Publication Abstract from PubMed In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation. Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis.,Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N Mol Cell. 2020 Aug 20;79(4):629-644.e4. doi: 10.1016/j.molcel.2020.06.030. Epub, 2020 Jul 16. PMID:32679035 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also GTP-binding protein 3D structures Pseudouridine synthase 3D structures Ribosome 3D structures

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